New colorimetric method with bromocresol purple for estimating the redox state of human serum albumin
نویسندگان
چکیده
منابع مشابه
Measurement of serum albumin by capillary zone electrophoresis, bromocresol green, bromocresol purple, and immunoassay methods.
BACKGROUND/AIMS The introduction of capillary zone electrophoresis (CZE) to this laboratory has highlighted discrepancies in albumin measured on an Abbott Aeroset by bromocresol green (BCG) and that calculated by CZE on the basis of total protein measured by Biuret. METHODS This study examined differences in albumin estimation by CZE, Abbott Aeroset BCG, and Aeroset bromocresol purple (BCP), ...
متن کاملBromocresol purple, a non-specific colour reagent for the determination of serum albumin.
The determination of serum albumin with the dye reagent bromocresol purple was investigated. We found that bromocresol purple is not a specific reagent for albumin, but that serum proteins in the alpha-, beta-, and gamma-globulin fractions also react with this dye. Similar results were obtained for bromocresol green. Furthermore there is a great difference in absorbance between human and bovine...
متن کاملThe introduction of bromocresol purple for the determination of serum albumin on SMAC and ACA, and the standardization procedure.
The bromocresol purple methods for the determination of serum albumin on the Technicon SMAC and the DuPont ACA systems were evaluated and compared with the corresponding bromocresol green methods, a radial immunodiffusion (RID) and an electrophoresis technique. The linearity and precision of the dye binding methods were comparable. The bromocresol purple methods were more influenced by lipaemia...
متن کاملNew automated dye-binding method for serum albumin determination with bromcresol purple.
We describe a new automated dye-binding method for serum albumin determination with bromcresol purple (BCP) that has several advantages over an existing bromcresol green (BCG) method. The continuous-flow method is sensitive, linear, and precise, with negligible sample interaction at an analytical rate of 60 samples per hour. Unlike BCG, BCP did not react with an albumin-free serum globulin prep...
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Human serum albumin (HSA) is an important protein that carries variety of substances like some hormones and drugs in blood. Pharmacological studies of the interaction of many drugs and HSA are reported during several decades, specially recently years. Interaction of cortisol and fluoxetine hydrochloride (FLX) (as a common anti-stress drug) with HSA (as their carrier in blood) has been studied s...
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ژورنال
عنوان ژورنال: Journal of Clinical Biochemistry and Nutrition
سال: 2020
ISSN: 0912-0009,1880-5086
DOI: 10.3164/jcbn.20-10